Urine analysis using FTIR spectroscopy: A study on healthy adults and children

SARIGÜL N., Kurultak İ., Uslu Gökceoğlu A., Korkmaz F.

Journal of Biophotonics, cilt.14, sa.7, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 7
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1002/jbio.202100009
  • Dergi Adı: Journal of Biophotonics
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, BIOSIS, Biotechnology Research Abstracts, Communication Abstracts, Compendex, EMBASE, INSPEC, MEDLINE, Metadex, Civil Engineering Abstracts
  • Anahtar Kelimeler: adult urine, band assignment, children urine, FTIR, normal variation, urine spectrum, TRANSFORM INFRARED-SPECTROSCOPY, HUMAN METABOLOME DATABASE, VIBRATIONAL SPECTROSCOPY, CITRATE EXCRETION, PHOSPHORUS, SERUM, HMDB, CREATININE, PHOSPHATE, DIAGNOSIS
  • Hacettepe Üniversitesi Adresli: Evet

Özet

© 2021 Wiley-VCH GmbH.Urine spectra from 108 healthy volunteers are studied by attenuated total refraction-Fourier transform infrared (ATR-FTIR) spectroscopy. The spectral features are correlated with observable urine components. The variation of spectra within a healthy population is quantified and a library of reference spectra is constructed. Using the band assignments, these spectra are compared with both age-wise and gender-wise. Children show the least intensity variations compared to both adult groups. Young adults show the highest variation, particularly in the 1650 to 1400 cm−1 and 1200 to 900 cm−1 regions. These results indicate the importance of the size of the control group in comparative studies utilizing FTIR. Age-wise comparisons reveal that phosphate and sulfate excretion decreases with age, and that the variance of phosphate among individuals is higher with adults. As for gender-wise comparisons, females show a slightly higher citrate content at 1390 cm−1 regardless of the age and they show a higher variance in the 1200 to 1000 cm−1 region when compared to men.