Study of lipoxygenase and peroxidase as blanching indicator enzymes in peas: change of enzyme activity, ascorbic acid and chlorophylls during frozen storage

GÖKMEN V., Bahceci K., Serpen A., Acar J.

LWT-FOOD SCIENCE AND TECHNOLOGY, vol.38, no.8, pp.903-908, 2005 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 38 Issue: 8
  • Publication Date: 2005
  • Doi Number: 10.1016/j.lwt.2004.06.018
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.903-908
  • Keywords: lipoxygenase., peroxidase, ascorbic acid, chlorophylls, blanching indicator, frozen storage, THERMAL INACTIVATION KINETICS, LIQUID-CHROMATOGRAPHIC METHOD, LIPID OXIDATION, BROCCOLI, QUALITY, REGENERATION, DEGRADATION, ASPARAGUS, BEANS, FRESH
  • Hacettepe University Affiliated: Yes


In this study, the effects of different blanching conditions on residual lipoxygenase (LOX) and peroxidase (POD) activities and quality changes in peas during frozen storage were studied. Peas were analysed for LOX and POD activities, ascorbic acid and chlorophylls a and b contents at 1, 2, 3, 6, 9 and 12 months of frozen storage. No regeneration of LOX and POD activities was determined in frozen-stored peas at -18 degrees C. The degradations of ascorbic acid and chlorophylls followed first-order kinetics. The half-lives of ascorbic acid, chlorophylls a and b derivatives in unblanched peas were found to be 3.30, 14.01 and 36.76 months during storage, respectively. Blanching at both 70 degrees C for 4.0 min and 80 degrees C for 2.0 min increased the half-life of ascorbic acid while it decreased those of chlorophylls a and b. Overall results suggested a blanching time of 2.0 min at 80 degrees C to inactivate 90% of initial POD activity, so, to retain quality parameters such as ascorbic acid and chlorophyll pigments in a storage period of 12 months at -18 degrees C. (c) 2004 Published by Elsevier Ltd. on behalf of Swiss Society of Food Science and Technology.