RP-HPLC Method Development for Determination of Curcumin in Commercial Turmeric Capsules

Eneş D., Çelebier M., DİKMEN D., Altinöz S.

Hacettepe University Journal of the Faculty of Pharmacy, vol.44, no.1, pp.1-8, 2024 (Scopus) identifier


Curcumin is a natural polyphenol product isolated from the rhizome of the Curcuma longa plant and is the most active ingredient of this plant. Curcumin is hepatoprotective, nephroprotective, anti-thrombotic, cardioprotective, anti-diabetic and anti-rheumatic. The aim of this study was to develop an HPLC method for the analysis of curcumin in commercially available capsules. Supelco Discovery 5 µm C18 Column (4.6mm x 5.0 µm) was used for chromatographic separation. The mobile phase was acetonitrile (ACN), 20 mM phosphate buffer, pH 6 (40:60 v/v). The flow rate was 1.5 mL min-1. UV detection was at 262 nm. The retention time was about 6.13th minutes for curcumin. The developed method has been validated according to ICH guidelines. The method was found to be selective under the experimental conditionsIt was linear over the range 0.05 - 50.0 μg mL-1. The intraday and interday studies showed that the RSD % was less than 2.0 for intraday and 2.0 for interday, and the Bias% was less than 2.0 for intraday and 2.0 for interday. The method could be successfully used in curcumin-loaded commercial capsules’ quality control.