DNA adsorption onto polyethylenimine-attached poly(p-chloromethylstyrene) beads


Unsal E., Bahar T., Tuncel M., Tuncel A.

JOURNAL OF CHROMATOGRAPHY A, cilt.898, sa.2, ss.167-177, 2000 (SCI İndekslerine Giren Dergi) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 898 Konu: 2
  • Basım Tarihi: 2000
  • Doi Numarası: 10.1016/s0021-9673(00)00831-1
  • Dergi Adı: JOURNAL OF CHROMATOGRAPHY A
  • Sayfa Sayıları: ss.167-177

Özet

In this study, DNA adsorption properties of polyethylenimine (PEI)-attached poly(p-chloromethylstyrene) (PCMS) beads were investigated. Spherical beads with an average size of 186 mum were obtained by the suspension polymerization of p-chloromethylstyrene conducted in an aqueous dispersion medium. Owing to the reasonably rough character of the bead surface, PCMS beads had a specific surface area of 14.1 m(2)/g. PEI chains could be covalently attached onto the PCMS beads with equilibrium binding capacities up to 208 mg PEI/g beads, via a direct chemical reaction between the amine and chloromethyl groups. After PEI adsorption with 10% (w/w) initial PEI concentration, free amino content of PEI-attached PCMS beads was determined as 0.91 mequiv./g. PEI-attached PCMS beads were utilized as sorbents in DNA adsorption experiments conducted at +4 degreesC in a phosphate buffer medium of pH 7.4. DNA immobilization capacities up to 290 mg DNA/g beads could be achieved with the tried sorbents, This value was approximately 50-times higher relative to the adsorption capacities of previously examined sorbents. (C) 2000 Elsevier Science B.V. All rights reserved.