Endothelial Dysfunction in the Mesenteric Artery and Disturbed Nonadrenergic Noncholinergic Relaxation of the Ileum due to Intestinal Ischemia-Reperfusion Can Be Prevented by Sildenafil

Soydan G., CEKIC E. G. , TUNCER M.

PHARMACOLOGY, vol.84, no.2, pp.61-67, 2009 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 84 Issue: 2
  • Publication Date: 2009
  • Doi Number: 10.1159/000225971
  • Journal Name: PHARMACOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.61-67
  • Hacettepe University Affiliated: Yes


The aim of the present study was to evaluate the effects of sildenafil on endothelium-dependent mesenteric artery vasorelaxation and nonadrenergic noncholinergic (NANC) ileal responses in an experimental rat intestinal ischemia-reperfusion (I/R) model. The superior mesenteric artery was occluded for 45 min of ischemia and then the clamp was removed for 60 min of reperfusion. Sildenafil (1 mg/kg, i.v.) or saline was administered prior to surgery in the I/R and sham-operated groups. Acetylcholine-induced relaxation of the mesenteric arteries, which were precontracted via sub-maximal phenylephrine, decreased markedly after I/R. Sildenafil pretreatment reversed the acetylcholine-induced relaxation. In the ileum, NANC responses were significantly attenuated following I/R, which were increased by sildenafil pretreatment. These results indicate that pretreatment with sildenafil prevented both endothelial dysfunction in the mesenteric artery and impairment of ileal NANC responses in a rat intestinal I/R model. Copyright (C) 2009 S. Karger AG, Basel