Multifunctional boronic acid-modified zirconium-based MOFs as peroxidase-mimicking colorimetric platforms for β-NAD capture and detection

Kapucu B. G., Parlak S., HACIEFENDİOĞLU D., DEMİR M., Tuncel A., Kip Ç.

Talanta, vol.308, 2026 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 308
  • Publication Date: 2026
  • Doi Number: 10.1016/j.talanta.2026.129854
  • Journal Name: Talanta
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, Chimica, Compendex
  • Keywords: Boronic acid functionalization, cis-diol recognition, Dual functionality, Nanozyme, Peroxidase-like activity, β-NAD detection
  • Hacettepe University Affiliated: Yes

Abstract

Metal–organic frameworks (MOFs) offer tunable porosity, large surface area, and versatile surface chemistry, making them promising candidates for multifunctional nanoplatforms. Here, we report the functionalization of zirconium based MOFs (UiO-66-NH2) with 4-formylphenylboronic acid (FPBA) to integrate cis -diol recognition capability with peroxidase-mimicking catalytic activity. Functionalization of UiO-66-NH2 with FPBA provides a versatile strategy for advanced MOF design, introducing cis -diol recognition sites while preserving the intrinsic porosity of the framework. The resulting UiO-66-NH2-FPBA exhibits high β-NAD adsorption capacity (78.2 mg g−1) and efficient desorption under neutral conditions. Moreover, its intrinsic catalytic activity enables colorimetric detection through OPDA oxidation, with kinetic parameters (Km = 1364.7 μM, Vm = 84.03 μM min−1) comparable to natural enzymes. The presented method is efficient, rapid, cost-effective, and sensitive for the colorimetric detection of β-NAD with a linear range from 0 to 750 μM in both acetate buffer, pH 5.5 (LOD = 2.9 μM) and diluted human serum (LOD = 30.2 μM). This dual functionality highlights the potential of FPBA-modified MOFs as advanced multifunctional nanoplatforms, bridging molecular recognition and nanozyme catalysis for future applications in biochemical sensing and nanobiotechnology.