Use of heteroduplex analysis to classify legionellae on the basis of 5S rRNA gene sequences

Pinar, AHMET; Ahkee, S; Miller, RD; Ramirez, JA; Summersgill, JT

doi:10.1128/jcm.35.6.1609-1611.1997

Use of heteroduplex analysis to classify legionellae on the basis of 5S rRNA gene sequences

Pinar A., Ahkee S., Miller R., Ramirez J., Summersgill J.

JOURNAL OF CLINICAL MICROBIOLOGY, sa.6, ss.1609-1611, 1997 (SCI-Expanded, Scopus)

Yayın Türü: Makale / Tam Makale
Basım Tarihi: 1997
Doi Numarası: 10.1128/jcm.35.6.1609-1611.1997
Dergi Adı: JOURNAL OF CLINICAL MICROBIOLOGY
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.1609-1611
Hacettepe Üniversitesi Adresli: Evet

Özet

Seventeen different species of Legionella, 12 serogroups of Legionella pneumophila, and 2 Legionella-like amoebal pathogens (LLAP1 and Sarcobium lyticum) were examined by heteroduplex analysis of PCR products of the 5S rRNA gene. Eight different banding patterns were identified, indicating that heteroduplex analysis of this gene can be used to classify these bacteria according to base substitutions between species. This classification may have future applications in clinical and epidemiological studies.