T-2 toxin induces cytotoxicity and disrupts tight junction barrier in SerW3 cells

KARACAOĞLU E., SELMANOĞLU G.

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, vol.56, pp.259-267, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 56
  • Publication Date: 2017
  • Doi Number: 10.1016/j.etap.2017.10.005
  • Journal Name: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.259-267
  • Keywords: Cytotoxicity, N-cadherin, Occludin, Sertoli cell barrier, TEER, T-2 toxin, BLOOD-TESTIS BARRIER, OVARIAN GRANULOSA-CELLS, METABOLITE HT-2 TOXIN, IN-VITRO, PROTEIN-SYNTHESIS, CADMIUM CHLORIDE, SERTOLI-CELLS, LEYDIG-CELLS, VERO CELLS, TOXICITY
  • Hacettepe University Affiliated: Yes

Abstract

T-2 toxin, which is produced in grain and grain products as a secondary metabolite by Fusarium species, is also potentially dangerous for human health. Up to date, no study was reported the cytotoxicity of T-2 toxin on SerW3 cells in the perspective of junctional barriers. This study focused on revealing the cytotoxic effects of T-2 on Sertoli cells associated with cell junctional barriers. In the present study, SerW3 cells were exposed to T-2 toxin at 12, 120 and 1200 ng/ml doses for 24 and 48 h. Cytotoxicity tests including cell viability (MTT), lactate dehydrogenase (LDH) cytotoxicity test and trypan blue exclusion assay were performed. Occludin, ZO-1, N-cadherin and beta-catenin were immunolabelled, expressions of occludin and N-cadherin were determined by western blotting. SerW3 cell barrier integrity was measured by transepithelial electrical resistance (TEER). Cytotoxicity caused by T-2 toxin increased in a dose dependent manner, expressions of proteins and TEER measurement decreased. This study may underlie the early targets of T-2 toxin on SerW3 cells mimicking blood testis barrier in vitro.