Biomimetic Apatite-coated PCL Scaffolds: Effect of Surface Nanotopography on Cellular Functions

Beskardes I. , Guemuesderelioglu M.

JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, cilt.24, ss.507-524, 2009 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 24 Konu: 6
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1177/0883911509349311
  • Sayfa Sayıları: ss.507-524


In this study, polycaprolactone (PCL) scaffolds, consisting of agglomerated microspheres with nanotopographic surface structures, were fabricated by the freeze-drying method. These scaffolds were coated with bone-like apatite by using a calcium phosphate solution similar to saturated simulated body fluid (10 x SBF-like) in two different immersion periods (6 and 24 h). Scanning electron microscopic views of the 6-h treatment in 10 x SBF-like solution showed formation of calcium phosphate nucleation sites on the PCL scaffolds, while the apatite particles formed characteristic cauliflower-like morphology after 24 h. The X-ray diffraction (XRD) data showed that the mineral phase was made of hydroxyapatite (HA). The osteogenic activity of untreated and SBF-treated PCL scaffolds was examined by pre-osteoblastic MC3T3 cell culture studies. Cells had attached and spread on both the PCL scaffolds and the 6-h SBF immersion-treated scaffolds.