Biomimetic apatite-coated PCL scaffolds: Effect of surface nanotopography on cellular functions

Beskardes I., Guemuesderelioglu M.

Journal of Bioactive and Compatible Polymers, vol.24, no.6, pp.507-524, 2009 (SCI-Expanded) identifier identifier


In this study, polycaprolactone (PCL) scaffolds, consisting of agglomerated microspheres with nanotopographic surface structures, were fabricated by the freeze-drying method. These scaffolds were coated with bone-like apatite by using a calcium phosphate solution similar to saturated simulated body fluid (10ã- SBF-like) in two different immersion periods (6 and 24 h). Scanning electron microscopic views of the 6-h treatment in 10ã- SBF-like solution showed formation of calcium phosphate nucleation sites on the PCL scaffolds, while the apatite particles formed characteristic cauliflower-like morphology after 24 h. The X-ray diffraction (XRD) data showed that the mineral phase was made of hydroxyapatite (HA). The osteogenic activity of untreated and SBF-treated PCL scaffolds was examined by pre-osteoblastic MC3T3 cell culture studies. Cells had attached and spread on both the PCL scaffolds and the 6-h SBF immersion-treated scaffolds.