DNA adsorption on a poly-L-lysine-immobilized poly(2-hydroxyethyl methacrylate) membrane


Senel S., Bayramoglu G., Arica M.

POLYMER INTERNATIONAL, vol.52, no.7, pp.1169-1174, 2003 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 52 Issue: 7
  • Publication Date: 2003
  • Doi Number: 10.1002/pi.1229
  • Journal Name: POLYMER INTERNATIONAL
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.1169-1174

Abstract

The DNA adsorption properties of poly-L-lysine-immobilized poly(2-hydroxyethyl methacrylate) (pHEMA) membrane were investigated. The pHEMA membrane was prepared by UV-initiated photopolymerization and activated with epichlorohydrin. Poly-L-lysine was then immobilized on the activated pHEMA membrane by covalent bonding, via a direct chemical reaction between the amino group of poly-L-lysine and the epoxy group of pHEMA. The poly-L-lysine content of the membrane was determined as 1537 mg m(-2). The poly-L-lysine-immobilized membrane was utilized as an adsorbent in DNA adsorption experiments. The maximum adsorption of DNA on the poly-L-lysine-immobilized pHEMA membrane was observed at 4 degreesC from phosphate-buffered salt solution (pH 7.4, 0.1 M; NaCl 0.5 M) containing different amounts of DNA. The non-specific adsorption of DNA on the plain pHEMA membrane was low (about 263 mg m(-2)). Higher DNA adsorption values (up to 5849 mg m(-2)) were obtained in which the poly-L-lysine-immobilized pHEMA membrane was used. (C) 2003 Society of Chemical Industry.