The Effects of One-Step Self-Etch Adhesives on the Induction of Oxidative Stress and Production of TGF-beta 1 and BMP-2 by Human Gingival Fibroblasts

Guven G., Cehreli S. B., Altun C., ÇEHRELİ Z. C., SERDAR M. A., Ural A. U.

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, vol.20, no.11, pp.1597-1610, 2009 (SCI-Expanded) identifier identifier identifier


The aim of this study was to evaluate and compare the effects of two self-etch adhesive materials on the induction of oxidative stress and production of transforming growth factor-beta 1 (TGF-beta 1) and bone morphogenetic protein-2 (BMP-2) by cultured human gingival fibroblasts (HGF). Inflammation-free attached gingiva was obtained from healthy donors under informed consent. Following 24- and 72-h exposure of HGF to two different elutes of the test materials, cell viability was determined using the 3-(4,5-dimethyl- 2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Lipid peroxidation, a major indicator of oxidative stress, was measured by the thiobarbituric acid reactive substance (TBARS) assay. TGF-beta 1 and BMP-2 levels in cell-free culture media were determined by enzyme-linked immunosorbent assay (ELISA). Cell viability of the test groups was significantly lower than those of control at 24 and 72 h (P < 0.001), but showed an increase at 72 h (P < 0.001). The TBARS levels of both test groups were significantly greater than that of control (P < 0.05), and displayed similar values at 72 h (P > 0.05). For both materials, the levels of TGF-beta 1 and BMP-2 were significantly greater than that of control (P < 0.05). Both test groups showed increased TGF-beta 1 levels. These results indicate that the tested self-etch adhesives might be capable of inducing production of TGF-beta 1 and BMP-2 in cultured HGF, despite their cytotoxic and oxidative stress-producing potential. (C) Koninklijke Brill NV, Leiden, 2009