LEPR, ADBR3, IRS-1 and 5-HTT genes polymorphisms do not associate with obesity


Mergen H., Karaaslan C., MERGEN M., Oezsoy E. D. , OEZATA M.

ENDOCRINE JOURNAL, vol.54, no.1, pp.89-94, 2007 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 54 Issue: 1
  • Publication Date: 2007
  • Doi Number: 10.1507/endocrj.k06-023
  • Journal Name: ENDOCRINE JOURNAL
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.89-94
  • Keywords: B-3-adrenergic receptor, insulin receptor substrate-1, serotonin transporter, leptin receptor, obesity, PCR, polymorphism, RFLP, SEROTONIN TRANSPORTER GENE, LEPTIN RECEPTOR GENE, BETA(3)-ADRENERGIC RECEPTOR, ANOREXIA-NERVOSA, INSULIN-RESISTANCE, DIABETES-MELLITUS, JAPANESE SUBJECTS, BODY-WEIGHT, MUTATION, SUBSTRATE-1

Abstract

Obesity is a growing problem and is associated with numerous medical conditions. In several genes coding for molecules involved in the regulation of body weight (fat mass) and thermogenesis, polymorphisms have been reported which possibly modify human obesity risk. The aim of this study was to determine the incidence of the following polymorphisms in the following genes in 262 obese (BMI :30) and 138 control (BMI: 25) subjects: leptin receptor (LEPR)-Gln223Arg, B-3-adrenergic receptor (B-3-AR)-Trp64Arg, serotonin transporter (5-HTT)-a 44-base pair insertion/ deletion functional polymorphism in the 5-HTTLPR and insulin receptor substrate-1 (IRS-1)-Gly972Arg. Our hypothesis was that these polymorphisms would occur more frequently in the obese population. The polymorphisms were determined by polymerase chain reaction (PCR) and restriction genotyping in study population. In our results, no strong associations were observed between BMI status and these polymorphisms. Weak, though significant, association coefficients obtained with HTT and LEPR loci indicate that the genotype numbers at these loci may depend on BM I status to some extent.