Gold-silver nanoclusters having dipicolinic acid imprinted nanoshell for Bacillus cereus spores recognition


Gultekin A., Diltemiz S. E. , Ersoz A., Sariozlu N. Y. , DENİZLİ A., SAY R.

TALANTA, vol.78, pp.1332-1338, 2009 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 78
  • Publication Date: 2009
  • Doi Number: 10.1016/j.talanta.2009.02.007
  • Journal Name: TALANTA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1332-1338
  • Hacettepe University Affiliated: Yes

Abstract

Molecular imprinted polymers (MIPs) as a recognition element for sensors are increasingly of interest and MIP nanoclusters have started to appear in the literature. in this study, we have proposed a novel thiol ligand-capping method with polymerizable methacryloylamidocysteine (MAC) attached to gold-silver nanoclusters, reminiscent of a self-assembled monolayer and have reconstructed surface shell by synthetic host polymers based on molecular imprinting method for recognition. In this method, methacryloyl iminodiacetic acid-chrome (MAIDA-Cr(III)) has been used as a new metal-chelating monomer via metal coordination-chelation interactions and dipicolinic acid (DPA) which is a main participant of Bacillus spores has been used as a template. Nanoshell sensors with templates give a cavity that is selective for DPA. The DPA can simultaneously chelate to Cr(III) metal ion and fit into the shape-selective cavity. Thus, the interaction between Cr(III) ion and free coordination spheres has an effect on the binding ability of the gold-silver nanoclusters nanosensor. The binding affinity of the DPA imprinted nanoclusters has been investigated by using the Langmuir and Scatchard methods and determined affinity constants (K-affinity) were found as 18 x 10(6) mol L-1 and 9 x 10(6) mol L-1, respectively. (C) 2009 Elsevier B.V. All rights reserved.