Molecularly imprinted cryogel cartridges for the selective recognition of tyrosine

Bakhshpour M., GÖKTÜRK BAŞAL I., BERELİ N., DENİZLİ A.

BIOTECHNOLOGY PROGRESS, cilt.36, sa.5, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 5
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/btpr.3006
  • Dergi Adı: BIOTECHNOLOGY PROGRESS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Applied Science & Technology Source, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, Computer & Applied Sciences, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: cryogel cartridges, hydrophobic interaction, metal-chelate coordination, molecular imprinting, tyrosine, L-GLUTAMIC ACID, SOL-GEL, CHROMATOGRAPHY, POLYMERS, PROTEIN, SENSOR, SITES, OXIDE
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Molecularly imprinted polymers are used for creating a specific cavity and selective recognition sites for the structure of a target molecule in a polymeric structure. In this study, specific molecularly imprinted cryogel cartridges were synthesized using two distinct functional monomers to compare imprinting efficiency for the selective recognition of Tyrosine (Tyr). Tyr-imprinted cryogel cartridge (MIP1) was prepared using metal-chelate coordination for the imprinting process by free-radical bulk polymerization under frozen conditions, and Tyr-imprinted cryogel cartridge (MIP2) was prepared in the same way using hydrophobic effects for imprinting. After the characterization of the cryogel cartridges was carried out, the optimum adsorption conditions of both were determined according to the different parameters such as flow rate (0.5-2.5 ml/min), pH of the medium (4.0-8.0), initial Tyr concentration (0.1-3.0 mg/ml), and temperature (4-45 degrees C). Selectivity experiments of Tyr-imprinted and non-imprinted cryogel cartridges were carried out by using phenylalanine, tryptophan, and cysteine. Besides, the eluted Tyr from MIP1 and MIP2 cryogel cartridge were applied to FPLC system. Also, the reusability experiments of Tyr-imprinted cryogel cartridges was observed no significant decrease in the adsorption capacity.