Akademik Veri Yönetim Sistemi
9th International Comet Assay Workshop (ICAW), Aydın, Türkiye, 13 - 16 Eylül 2011, ss.55
The single cell gel
electrophoresis or Comet assay is a visual, sensitive, relatively inexpensive
and noninvasive technique for quantitating DNA damage and repair in vivo and in
vitro in any eukaryotic cell and some prokaryotic cells. It also has an
advantage over the other DNA damage-detecting methods, such as micronucleus
assay, sister chromatid exchange and alkali elution, because of its high
sensitivity. However, the Comet assay has gained increasing popularity for use
in human biomonitoring, genotoxic, and cell biological studies. These studies
have shown that the parameters of tail moment and among other comet parameters
such as comet length, comet intensity, head length, head intensity, tail
length, tail intensity, tail DNA and olive tail moment were generally highly
correlated. Among these parameters of Comet features have been developed.
Especially, tail parameters that are the most frequently used are those of the
tail moment, the tail DNA, and the tail length, moreover these parameters have
been used by many researchers for radiation studies, but the tail moment and
tail DNA showed more sensitivity than the tail length. Nevertheless, the tail
DNA was more accurate than the tail moment in comparing the sensitivity of the
alkaline and neutral assays in an experimental cell study, it is of interest to
investigate which parameter is more suitable and safer for measuring DNA
damages in a human biomonitoring study. The reliability of the results in terms
of parameters of the tail moment and the tail DNA studies should be evaluated
together.