Cibacron blue immobilized poly(glycidyl-methacrylate) nanobeads for albumin removal in proteome studies


Andac M.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, cilt.43, ss.133-139, 2015 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 43 Konu: 2
  • Basım Tarihi: 2015
  • Doi Numarası: 10.3109/21691401.2013.852102
  • Dergi Adı: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Sayfa Sayıları: ss.133-139

Özet

A new approach to albumin removal for proteome studies involving human plasma samples was presented with dye affinity poly glycidyl methacrylate (PGMA) nanobeads in an average size of 45 nm. The specific surface area of PGMA nanobeads was calculated as 2616 m(2)/g. Cibacron Blue F3GA (CB) was immobilized onto PGMA nanobeads as dye ligand, and CB immobilized PGMA (CB-PGMA) nanobeads were characterized by a serial processing. Albumin depletion efficiency of CB-PGMA nanobeads was investigated in human plasma sample and confirmed by two-dimensional gel electrophoresis.