THE TALE OF TWO PROTEINS: Interaction Between Desmin and Lamin B

Kural Mangıt E., Dinçer P. R.

6th International Congress of the Molecular Biology Association of Turkey, İzmir, Türkiye, 05 Eylül 2018, ss.16

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: İzmir
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.16
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Desmin is a muscle specific intermediate filament protein located in cytoplasm. Lamin B, on the other hand, is a nuclear intermediate filament protein. In this study the interaction between desmin and lamin B has been investigated. Proteins of interest were isolated from muscle tissue of wild type AB zebrafish and the interaction was determined via reciprocal co-immunoprecipitation (Co-IP). To confirm the results proximity ligation assay was used in skeletal muscle sections of zebrafish and a mass spectrometry (MS) analysis was performed on Co-IP lysates.

The results of MS analysis also showed that there is an interaction between desmin and Nup153, a FG Nup located in the central channel of the nuclear pore complex that is responsible for controlling nucleocytoplasmic transport, which is  known to interact with lamin B. Also the Co-IP results revealed an interaction between desmin and Nup214 -another FG Nup. All together this data suggests that desmin might enter the nucleus to regulate one or more cellular processes in muscle cells.

 

This project is funded  by TUBITAK (Project no: 214S174).