A new amperometric enzyme electrode for galactose determination

Gulce H., Ataman I., Gulce A., Yildiz A.

ENZYME AND MICROBIAL TECHNOLOGY, cilt.30, sa.1, ss.41-44, 2002 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 1
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1016/s0141-0229(01)00452-5
  • Dergi Adı: ENZYME AND MICROBIAL TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.41-44
  • Hacettepe Üniversitesi Adresli: Hayır

Özet

A new enzyme electrode for the determination of galactose was developed by immobilizing galactose oxidase in polyvinylferrocenium matrix coated on a Pt electrode surface. The amperometric response of the enzyme electrode was measured at a constant potential of +0.70V vs SCE. The effects of galactose concentration and temperature on the response of the enzyme electrode were investigated. The response time was found to be 30-40 s and the upper limit of the linear working portion was found to be 40.0 mM galactose concentration. (C) 2002 Elsevier Science Inc. All rights reserved.