A new amperometric enzyme electrode for galactose determination

Gulce, H; Ataman, I; Gulce, A; Yildiz, A

doi:10.1016/s0141-0229(01)00452-5

A new amperometric enzyme electrode for galactose determination

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Gulce H., Ataman I., Gulce A., Yildiz A.

ENZYME AND MICROBIAL TECHNOLOGY, vol.30, no.1, pp.41-44, 2002 (SCI-Expanded)

Publication Type: Article / Article
Volume: 30 Issue: 1
Publication Date: 2002
Doi Number: 10.1016/s0141-0229(01)00452-5
Journal Name: ENZYME AND MICROBIAL TECHNOLOGY
Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Page Numbers: pp.41-44
Hacettepe University Affiliated: No

Abstract

A new enzyme electrode for the determination of galactose was developed by immobilizing galactose oxidase in polyvinylferrocenium matrix coated on a Pt electrode surface. The amperometric response of the enzyme electrode was measured at a constant potential of +0.70V vs SCE. The effects of galactose concentration and temperature on the response of the enzyme electrode were investigated. The response time was found to be 30-40 s and the upper limit of the linear working portion was found to be 40.0 mM galactose concentration. (C) 2002 Elsevier Science Inc. All rights reserved.