A new amperometric enzyme electrode for galactose determination


Gulce H., Ataman I., Gulce A., Yildiz A.

ENZYME AND MICROBIAL TECHNOLOGY, vol.30, no.1, pp.41-44, 2002 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 1
  • Publication Date: 2002
  • Doi Number: 10.1016/s0141-0229(01)00452-5
  • Title of Journal : ENZYME AND MICROBIAL TECHNOLOGY
  • Page Numbers: pp.41-44

Abstract

A new enzyme electrode for the determination of galactose was developed by immobilizing galactose oxidase in polyvinylferrocenium matrix coated on a Pt electrode surface. The amperometric response of the enzyme electrode was measured at a constant potential of +0.70V vs SCE. The effects of galactose concentration and temperature on the response of the enzyme electrode were investigated. The response time was found to be 30-40 s and the upper limit of the linear working portion was found to be 40.0 mM galactose concentration. (C) 2002 Elsevier Science Inc. All rights reserved.