Molecularly imprinted label-free sensor platform for impedimetric detection of 3-monochloropropane-1,2-diol

YAMAN Y. T., BOLAT G., Saygin T. B., ABACI S.

SENSORS AND ACTUATORS B-CHEMICAL, vol.328, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 328
  • Publication Date: 2021
  • Doi Number: 10.1016/j.snb.2020.128986
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Analytical Abstracts, Biotechnology Research Abstracts, Chemical Abstracts Core, Communication Abstracts, INSPEC, Metadex, Pollution Abstracts, Civil Engineering Abstracts
  • Keywords: 3-MCPD, Impedimetric sensor, Molecularly imprinted polymer, Graphene oxide, Food control, GLASSY-CARBON ELECTRODE, ELECTROCHEMICAL SENSOR, GRAPHENE-OXIDE, SELECTIVE RECOGNITION, POLYPYRROLE, 3-MCPD, ACID, WATER, FOOD, SOY
  • Hacettepe University Affiliated: Yes


The fabrication of a molecularly imprinted overoxidized polypyrrole MIP(oPPy) on graphene oxide (GO) modified single-use pencil graphite electrode (PGE) for the quantitative analysis of 3-monochloropropane-1,2-diol (3-MCPD), an important food processing contaminant, was reported for the first time. In situ electropolymerization of oPPy in the presence of template molecules (3-MCPD) onto GO/PGE was carried out via cyclic voltammetry method in a pH-controlled solution. To evaluate the most appropriate sensor response, various experimental conditions (such as film thickness, analyte/monomer ratio, desorption/adsorption time etc.) were discussed and optimized. The re-adsorption of 3-MCPD was screened by electrochemical impedance spectroscopy (EIS) on imprinted cavities capable of 3-dimensional recognition of the target molecule. The electrochemistry of a redox probe (ferrocyanide/ferricyanide) was utilized for the impedimetric detection of 3-MCPD with a dynamic linear range of 2 500 nM and low sensing limit as 1.82 nM. The combination of GO and oPPy to construct a MIP platform provided a superior recognition performance for 3-MCPD in a label-free, disposable and cost-effective way. In order to demonstrate the practical applicability, standard addition method was employed in soy sauce samples. The achieved high recovery rates displayed that MIP (oPPy)-GO/PGE hold significant promise for future on-site studies in real food matrices for food safety control purposes.