Cibacron Blue F3GA incorporated poly(methylmethacrylate) beads for albumin adsorption in batch system


Yavuz H. , DURU E., GENC O., DENIZLI A.

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, cilt.223, ss.185-193, 2003 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 223
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1016/s0927-7757(03)00153-5
  • Dergi Adı: COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
  • Sayfa Sayıları: ss.185-193

Özet

A new adsorbent was prepared to obtain high albumin adsorption capacity utilizing Cibacron Blue F3GA as a dye ligand. Poly(methylmethacrylate) (PMMA) beads (100-150 mum in diameter) were produced by suspension polymerization. Ester groups in the PMMA structure were converted to imine groups by reacting with poly(ethylene imine) (PEI) in the presence of NaH. Then, dye ligand, Cibacron Blue F3GA, was covalently attached onto PMMA/ PEI beads for human serum albumin (HSA) adsorption from both aqueous solutions and human plasma. Cibacron Blue F3GA loading was found to be 95.8 mumol g(-1). HSA adsorption onto PM MA, PM MA/PEI and Cibacron Blue F3GA-attached PMMA/PEI beads was investigated batchwise. The adsorption of HSA onto PMMA was low (6.0 mg g(-1)). Cibaron Blue F3GA attachment onto the beads increased significantly the HSA adsorption (26.0 mg g(-1)). The maximum HSA adsorption was observed at pH 5.0. Higher HSA adsorption was observed from human plasma (64.3 mg HSA g(-1)). Adsorption of other serum proteins were found to be 2.8 mg g(-1) for fibrinogen and 5.3 mg g(-1) for gamma-globulins. The total protein adsorption was determined as 73.1 mg g(-1). Desorption of HSA was obtained using 0.5 M KSCN solution. High desorption ratios (up to 98% of the adsorbed HSA) were observed. It was possible to reuse Cibacron Blue F3GA-attached PMMA/PEI beads without significant decrease in the HSA adsorption capacity. (C) 2003 Published by Elsevier B.V.