Important role of milk in human, especially in infant nutrition is well known. On the other hand, contamination of milk with aflatoxins is considered as a potential risk for human health. The aim of this study was to determine the levels of aflatoxin M-1 in commonly consumed milk samples in Ankara, capital city of Turkey. Aflatoxin M-1 levels were investigated by high performance liquid chromatography (HPLC) with a fluorescence detector following sample clean-up using immunoaffinity columns. The mean recovery of the method was 117.9%. Standard curves were linear in the range of 10-200 ng/l with correlation coefficient of 0.9998. The limit of detection was found to be 10 ng/l. In this study, 27 milk samples were analysed. 24 of them were in categories of ultra high temperature treated, and 3 of them were daily-pasteurised milk. Aflatoxin M-1 was detected in 59.3% of all samples. However, only one sample among these was contaminated at a level above the maximum permissible limit (50 ng/l) accepted by European Union and Turkey for aflatoxin M-1. (c) 2004 Elsevier Ltd. All rights reserved.