Assessment of aflatoxin M-1 levels in milk in Ankara, Turkey

Gurbay A., Aydin S., Girgin G., Engin A., Sahin G.

FOOD CONTROL, vol.17, no.1, pp.1-4, 2006 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 17 Issue: 1
  • Publication Date: 2006
  • Doi Number: 10.1016/j.foodcont.2004.07.008
  • Journal Name: FOOD CONTROL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1-4
  • Keywords: milk, aflatoxin M-1, high performance liquid chromatography, immunoaffinity column, DAIRY-PRODUCTS, METABOLISM, HPLC
  • Hacettepe University Affiliated: Yes


Important role of milk in human, especially in infant nutrition is well known. On the other hand, contamination of milk with aflatoxins is considered as a potential risk for human health. The aim of this study was to determine the levels of aflatoxin M-1 in commonly consumed milk samples in Ankara, capital city of Turkey. Aflatoxin M-1 levels were investigated by high performance liquid chromatography (HPLC) with a fluorescence detector following sample clean-up using immunoaffinity columns. The mean recovery of the method was 117.9%. Standard curves were linear in the range of 10-200 ng/l with correlation coefficient of 0.9998. The limit of detection was found to be 10 ng/l. In this study, 27 milk samples were analysed. 24 of them were in categories of ultra high temperature treated, and 3 of them were daily-pasteurised milk. Aflatoxin M-1 was detected in 59.3% of all samples. However, only one sample among these was contaminated at a level above the maximum permissible limit (50 ng/l) accepted by European Union and Turkey for aflatoxin M-1. (c) 2004 Elsevier Ltd. All rights reserved.