An Overview of In Vitro Release Test Methods Used In The Study of Nanoparticular Drug Delivery Systems Nanopartiküler İlaç Taşıyıcı Sistemlerinin İncelenmesinde Kullanılan İn Vitro Salım Testi Yöntemlerine Genel Bir Bakış

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Çobanoğlu E., ŞENEL S.

Hacettepe University Journal of the Faculty of Pharmacy, vol.43, no.1, pp.78-98, 2023 (Scopus) identifier

  • Publication Type: Article / Review
  • Volume: 43 Issue: 1
  • Publication Date: 2023
  • Doi Number: 10.52794/hujpharm.1181365
  • Journal Name: Hacettepe University Journal of the Faculty of Pharmacy
  • Journal Indexes: Scopus
  • Page Numbers: pp.78-98
  • Keywords: continuous flow method, in vitro release test methods, membrane diffusion method, nanoparticulate systems, sample and separate method
  • Hacettepe University Affiliated: Yes


The in vitro release tests which examine the release properties of the drug from the dosage form provide the opportunity to investigate the in vitro availability of the drug product as well as to compare its equivalency to other products. In order to obtain accurate and reliable results from in vitro release tests, it is crucial to select the right release test method and suitable release conditions (such as temperature, release medium, pH, agitation/flow rate, etc.). Although there are compendial in vitro release test methods available for various dosage forms, up to date there are no tests described for nanoparticulate systems in any pharma-copeia. In this review, the methods (such as sampling and separation, membrane diffusion, continuous flow, etc.) applied to test the in vitro drug release from nanoparticulate systems will be reviewed. The recent applications of these methods in different particulate systems will be summarized and the advantages and limitations of these test methods will be discussed.