De novo cloning and functional characterization of potassium channel genes and proteins in the crayfish Astacus leptodactylus (Eschscholtz, 1823) (Decapoda: Astacidea: Astacidae)

ERGİN B., SAĞLAM B., TAŞKIRAN Z. E., BAŞTUĞ T., PURALI N.

JOURNAL OF CRUSTACEAN BIOLOGY, cilt.42, sa.1, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 42 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1093/jcbiol/ruac018
  • Dergi Adı: JOURNAL OF CRUSTACEAN BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Animal Behavior Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Environment Index, Veterinary Science Database
  • Anahtar Kelimeler: Cherax quadricarinatus, Crustacea, heterologous expression, topology analysis, voltage clamp, STRETCH-RECEPTOR NEURONS, K+ CHANNEL, CRYSTAL-STRUCTURE, STRUCTURAL BASIS, ION-CHANNEL, EXPRESSION, CONDUCTANCE, CURRENTS, LOBSTER, PIP2
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Current knowledge about the molecular properties of the crustacean ion channels is rather limited even if crustaceans have been widely used as a model in neuroscience. We cloned for the first time two different potassium channel genes from the freshwater crayfish Astacus leptodactylus (), one of the genes functionally expressed in the Xenopus oocytes. The open-reading frames of the genes were 1,203 and 3,447 bp, respectively. The nucleic acid sequence of the genes and associated proteins were similar to those of a typical potassium channel. BLAST analyses indicated that one of the cloned genes had a substantial similarity to an inward-rectifier potassium channel whereas the other gene was similar to a high-conductance-KCa type potassium channel reported in related species. Transmembrane topology and three-dimensional structure of the coded proteins were calculated and functional regions of the channel proteins responsible for ion selectivity, voltage sensing, gating, and calcium binding were identified. One of the cloned channel genes has been expressed in the Xenopus oocytes. Analysis of the expressed potassium currents confirmed that the cloned gene was coding a typical Kir-type potassium channel with ATP sensitivity.