Assessment of the cytotoxic, genotoxic, and antigenotoxic potential of Pycnogenol (R) in in vitro mammalian cells

Taner G., Aydin S., Aytac Z., BAŞARAN A. A., Basaran N.

FOOD AND CHEMICAL TOXICOLOGY, cilt.61, ss.203-208, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 61
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1016/j.fct.2013.06.053
  • Dergi Adı: FOOD AND CHEMICAL TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.203-208
  • Anahtar Kelimeler: Pycnogenol (R), Neutral red uptake assay, Cytokinesis-block micronucleus assay, Alkaline comet assay, Trolox equivalent antioxidant capacity, PINE BARK EXTRACT, DNA-DAMAGE, HYDROGEN-PEROXIDE, FREE-RADICALS, ANTIOXIDANT DEFENSES, DIETARY-SUPPLEMENTS, PHENOLIC-COMPOUNDS, PLANT POLYPHENOLS, HUMAN-LYMPHOCYTES, OXIDATIVE DAMAGE
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Pycnogenol (R) (PYC), a standardized plant extract obtained from the bark of the French maritime pine Pious pinaster, has been suggested to exert strong antioxidant activity and used as a phytochemical remedy for various diseases. In this study, we investigated the antioxidant capacity of PYC by the trolox equivalent antioxidant capacity (TEAC) assay and the cytotoxicity by neutral red uptake (NRU) test in Chinese Hamster Ovary (CHO) cells. The genotoxic and antigenotoxic effects of PYC were evaluated by the cytokinesis-blocked micronucleus (CBMN) and alkaline comet assays in human peripheral blood lymphocytes. At the concentrations of 2-200 mu g/ml, PVC was found to have antioxidant activity. The viability of CHO cells during 24 h exposure were not affected at the concentrations of 5-150 mu g/ml of PVC. IC50 value of PVC was found to be 285 mu g/ml. At the concentrations above 100 mu g/ml, PYC alone induced DNA damage and increased MN frequency, although PVC at all concentrations in a dose dependent manner revealed a reduction in the frequency of MN and the extent of DNA damage induced by H2O2. These results suggest PYC might reduce H2O2 induced chromosome breakage and loss and DNA damage in cultured human lymphocytes. (C) 2013 Elsevier Ltd. All rights reserved.