Recognition of lysozyme using surface imprinted bacterial cellulose nanofibers

SAYLAN Y., Tamahkar E., DENİZLİ A.

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, vol.28, no.16, pp.1950-1965, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 16
  • Publication Date: 2017
  • Doi Number: 10.1080/09205063.2017.1364099
  • Journal Name: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1950-1965
  • Keywords: Bacterial cellulose nanofibers, lysozyme recognition, metal ion coordination, protein purification, surface imprinting, QUARTZ-CRYSTAL MICROBALANCE, SILICA NANOPARTICLES, COMPOSITE CRYOGELS, BOVINE HEMOGLOBIN, PURIFICATION, PROTEIN, ADSORPTION, MEMBRANES, AFFINITY, SEPARATION
  • Hacettepe University Affiliated: Yes

Abstract

Here, we developed the lysozyme imprinted bacterial cellulose (Lyz-MIP/BC) nanofibers via the surface imprinting strategy that was designed to recognize lysozyme. This study includes the molecular imprinting method onto the surface of bacterial cellulose nanofibers in the presence of lysozyme by metal ion coordination, as well as further characterizations methods FTIR, SEM and contact angle measurements. The maximum lysozyme adsorption capacity of Lyz-MIP/BC nanofibers was found to be 71mg/g. The Lyz-MIP/BC nanofibers showed high selectivity for lysozyme towards bovine serum albumin and cytochrome c. Overall, the Lyz-MIP/BC nanofibers hold great potential for lysozyme recognition due to the high binding capacity, significant selectivity and excellent reusability.