Purification and kinetics of sheep kidney cortex glucose-6-phosphate dehydrogenase


Ulusu N., Tandogan B.

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, cilt.143, ss.249-255, 2006 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 143 Konu: 2
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1016/j.cbpb.2005.11.018
  • Dergi Adı: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
  • Sayfa Sayıları: ss.249-255

Özet

Glucose-6-phosphate dehydrogenase (G-6-PD) is one of the important enzymes, which is responsible for the production of NADPH and ribose-5-phosphate. NADPH is used for the biosynthetic reactions and protection of the cells from free radicals. We have investigated some properties and kinetic mechanism of the sheep kidney cortex G-6-PD. This enzyme has been purified 1384-fold with a yield of 16.96% and had a specific activity of 27.69 U/mg protein. The purification procedure consists of 2', 5'-ADP-Sepharose 4B affinity chromatography after ultracentrifugation. The sheep kidney cortex G-6-PD was found to operate according to a Ping Pong Bi Bi mechanism. The kinetic parameters from sheep K-m values for G-6-P and NADP(+) and V-m were determined to be 0.041 +/- 0.0043 mM, 0.0147 +/- 0.001 mM and 28.23 +/- 0.86 mu Mol min(-1) mg protein(-1) respectively. The pH optimum was 7.4 and the optimum temperature was 45 degrees C. In our previous study we have found that lamb kidney cortex G-6-PD enzyme obeys 'Ordered Bi Bi' mechanism. We suggest that kinetic mechanism altered due to the aging since sheep G-6-PD uses a 'ping pong' mechanism. (C) 2005 Elsevier Inc. All rights reserved.