Preparation and characterization of bFGF and BSA-loaded microspheres


Cetin M., Capan Y., Vural I. , Dogan A., Guc D. , Hincal A., ...Daha Fazla

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, cilt.15, ss.371-375, 2005 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 15 Konu: 5
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/s1773-2247(05)50067-4
  • Dergi Adı: JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
  • Sayfa Sayıları: ss.371-375

Özet

This article describes the preparation and characterization of BSA and bFGF-loaded PLGA microspheres and assesses the effect of entrapment procedure and in vitro release conditions. In the experiments, changing the PVA concentration from 3 to 5% (w/v) in the pre-emulsion innerphase led to a change in particle size and encapsulation efficiency of BSA-loaded and bFGF-loaded PLGA microspheres from 1 to 0.7 mu m and from 73 to 65%, respectively. The in vitro release of the bFGF from PLGA microspheres was much lower than BSA-loaded PLGA microspheres. For all of the formulations, BSA and bFGF retained integrity after the microencapsulation process as evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Besides, the cell proliferation-stimulating activity of bFGF was evaluated by using BALB/c 3T3 cells and it was shown that the bioactivity of bFGF was increased by adding heparin to the release medium.