Akademik Veri Yönetim Sistemi
MICROCHEMICAL JOURNAL, 2025 (SCI-Expanded)
Cyanide poisoning presents a significant threat to human health, often overlooked in emergency settings. The lack of portability and ease of use of traditional detection methods requires the development of fast and accessible alternatives. Here, we introduce smartphone-paired microfluidic sensing platform for rapid cyanide detection from whole human blood. This platform combines the use of fluorescent gold nanoclusters (AuNCs) with a compact disc (CD)-based centrifugal microfluidic system. Bovine Serum Albumin (BSA)-stabilized AuNCs demonstrate high selectivity and sensitivity towards cyanide, enabling simple colorimetric detection even in complex solutions. The microfluidic CD design allows for rapid plasma separation, enhancing the detection efficiency in one step. Sensitivity tests revealed a detection range of 0.1 mg L- 1 to 5 mg L- 1 cyanide in human plasma, within safe and dangerous intervals described in literature. Furthermore, the system exhibits high specificity to cyanide over other metal ions and anions. Smartphone camera analysis, utilizing the L*a*b* color system, provides a user-friendly interface for rapid detection. The developed system offers advantages of portability, rapid analysis time, and simplicity compared to traditional methods. Its potential for point-of-care testing in emergency situations, including fires and accidents, marks a significant advancement in cyanide detection technology. Overall, this platform presents a promising solution for rapid and accurate cyanide detection in various real-world scenarios, including environmental monitoring and emergency medical care. It achieves a limit of detection (LOD) of 250 nM in aqueous solutions, which is 10.8 times lower than the World Health Organization's (WHO) permissible limit of 2700 nM for drinking water. In human plasma, it demonstrates a LOD of 100 ng mL- 1, which is five times lower than the reported toxic cyanide dose of 500 ng mL- 1 in the human body.