In this study the relationship between pre-service chemistry teachers' beliefs about teaching and their pedagogical content knowledge were investigated. The sample of the study consists of 99 pre-service chemistry teachers attending Hacettepe University, Faculty of Education. As data collection tools the adapted form of "Beliefs About Teaching Scale" (BAT) developed by Yilmaz Tuzun (2008) and the "Pedagogical Content Knowledge Test" (PCK) were used. In order to determine the effect of gender on pre-service chemistry teachers' beliefs, independent sample t-test was conducted and no significant difference was observed between male and female pre-service teachers. And in order to determine the correlation between BAT and PCK scores correlation analysis were conducted and results revealed that the correlation between these two variables is not significant.