Isolation of anti-ulcerogenic sesquiterpene lactones from Centaurea solstitialis L. ssp solstitialis through bioassay-guided fractionation procedures in rats


Yesilada E., Gurbuz I., Bedir E., Tatli I., Khan W.

JOURNAL OF ETHNOPHARMACOLOGY, cilt.95, ss.213-219, 2004 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 95
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1016/j.jep.2004.07.021
  • Dergi Adı: JOURNAL OF ETHNOPHARMACOLOGY
  • Sayfa Sayıları: ss.213-219

Özet

The fresh spiny flowers of Centaurea solstitialis ssp. solstitialis (CSS) are used for the treatment of peptic ulcers in Turkey. Ethanol (80%) extract of CSS exhibited significant anti-ulcerogenic effect on the ethanol-induced ulcerogenesis model in rats. The ethanol extract was further fractionated by successive solvent extractions with n-hexane, chloroform, ethyl acetate and n-butanol. All fractions showed significant anti-ulcerogenic activity, however, the effect of the chloroform fraction was found to be more prominent with 99.5% ulcer inhibition. Bioassay-guided fractionation yielded sesquiterpene lactones as the active components. The main components responsible for the activity of the chloroform fraction were determined as chlorojanerin and 13-acetyl solstitialin A which were elucidated by HR-ESI and H-1, C-13 and 2D NMR spectroscopic techniques. (C) 2004 Elsevier Ireland Ltd. All rights reserved.