The effect of captopril on membrane bound enzymes in ischemia–reperfusion injury

Isbir C. S., DOĞAN R., Farsak B., Aydin M.

Vascular, vol.8, no.3, pp.192-197, 2000 (Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 8 Issue: 3
  • Publication Date: 2000
  • Doi Number: 10.1177/096721090000800304
  • Journal Name: Vascular
  • Journal Indexes: Scopus
  • Page Numbers: pp.192-197
  • Keywords: Ca2+/Mg2+ ATPase, captopril, cardioplegia, ischemia—reperfusion injury, myocardial protection, Na+ K+/Mg2+ ATPase
  • Hacettepe University Affiliated: Yes


There is substantial evidence that Na+ K+/Mg2+ ATPase and Ca2+/Mg2+ ATPase enzymes would effect the membrane integrity. Forty guinea pig (n = 10 in each group) hearts were studied in an isolated Krebs—Henseleit solution perfused Langendorff cardiac model. The first group was utilized as the control group. Group 2 hearts were arrested with captopril (200 μmol/l) added St Thomas Hospital Cardioplegic Solution (STHCS). Group 3 animals were pretreated with oral captopril (0.3 mg/kg/twice a day) for 10 days and then arrested with STHCS. Group 4 hearts were again pretreated with oral captopril (0.3 mg/kg/twice a day for 10 days) arrested with STHCS and reperfused with captopril added Krebs—Henseleit solution (200 μmoi/l). Hearts were subjected to normothermic global ischemia for 90 min and than were reperfused at 37°C. When the treated groups were compared with control, best results were achived by group 4. The Na+ K+ and Ca2+/Mg2+ ATPase levels increased from 466.38 ± 5.99 to 564.13 ± 7.77 and 884.69 ± 9.13 to 1254.29 ± 5.75 nmol Pi/mg/prot/h respectively (P < 0.05). These results suggest that captopril protects the membrane integrity and thus played a role at the recovery of depressed membrane bound Na+ K+ /Mg2+ ATPase and Ca2+/Mg2+ ATPase activity and also in ischemia—reperfusion injury. © 2000, The International Society for Cardiovascular Surgery. All rights reserved.