Molecularly imprinted cryogel columns for Concanavalin A purification from jack bean extract

ÇİMEN D., BERELİ N., Andac M., DENİZLİ A.

SEPARATION SCIENCE PLUS, cilt.1, sa.6, ss.454-463, 2018 (ESCI) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1 Sayı: 6
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1002/sscp.201800039
  • Dergi Adı: SEPARATION SCIENCE PLUS
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI)
  • Sayfa Sayıları: ss.454-463
  • Anahtar Kelimeler: Concanavalin A, cryogels, molecular imprinting, SUPERMACROPOROUS CRYOGELS, RECOGNITION, OLIGOSACCHARIDE, CHROMATOGRAPHY, ANTIBODIES, POLYMERS, REMOVAL, BINDING, SURFACE
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Concanavalin A, one of most studied lectins for the purification of glycoproteins and sugars, is selected as a model protein. In this study, a Concanavalin A imprinted poly(hydroxyethyl methacrylate) supermacroporous cryogel was prepared for the purification of Concanavalin from jack bean extract. N-Methacryloyl-L-histidine methyl ester with nickel(II) ions was used as the metal ion coordination complex. Concanavalin A imprinted cryogels were characterized by swelling degree, surface area, Fourier transform infrared spectroscopy, scanning electron microscopy and micro computed tomography measurements. Concanavalin A rebinding and desorption on Concanavalin A imprinted and non-imprinted cryogels were measured using a continuous system. Selective binding studies were carried out in the presence of Concanavalin B and bovine serum albumin. The selectivity studies were confirmed by fast protein liquid chromatography studies using jack bean extract from Canavalia ensiformis.