Molecularly imprinted cryogel columns for Concanavalin A purification from jack bean extract

ÇİMEN D., BERELİ N., Andac M., DENİZLİ A.

SEPARATION SCIENCE PLUS, vol.1, no.6, pp.454-463, 2018 (ESCI) identifier

  • Publication Type: Article / Article
  • Volume: 1 Issue: 6
  • Publication Date: 2018
  • Doi Number: 10.1002/sscp.201800039
  • Journal Name: SEPARATION SCIENCE PLUS
  • Journal Indexes: Emerging Sources Citation Index (ESCI)
  • Page Numbers: pp.454-463
  • Keywords: Concanavalin A, cryogels, molecular imprinting, SUPERMACROPOROUS CRYOGELS, RECOGNITION, OLIGOSACCHARIDE, CHROMATOGRAPHY, ANTIBODIES, POLYMERS, REMOVAL, BINDING, SURFACE
  • Hacettepe University Affiliated: Yes

Abstract

Concanavalin A, one of most studied lectins for the purification of glycoproteins and sugars, is selected as a model protein. In this study, a Concanavalin A imprinted poly(hydroxyethyl methacrylate) supermacroporous cryogel was prepared for the purification of Concanavalin from jack bean extract. N-Methacryloyl-L-histidine methyl ester with nickel(II) ions was used as the metal ion coordination complex. Concanavalin A imprinted cryogels were characterized by swelling degree, surface area, Fourier transform infrared spectroscopy, scanning electron microscopy and micro computed tomography measurements. Concanavalin A rebinding and desorption on Concanavalin A imprinted and non-imprinted cryogels were measured using a continuous system. Selective binding studies were carried out in the presence of Concanavalin B and bovine serum albumin. The selectivity studies were confirmed by fast protein liquid chromatography studies using jack bean extract from Canavalia ensiformis.