Vancomycin Loaded Gelatin Microspheres Containing Wet Spun Poly(epsilon-caprolactone) Fibers and Films for Osteomyelitis Treatment

AKSOY E. A., Yagci B. S., Manap G., EROĞLU İ., ÖZTÜRK Ş., EKİZOĞLU M., ...Daha Fazla

FIBERS AND POLYMERS, cilt.20, sa.11, ss.2236-2246, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 11
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1007/s12221-019-9271-7
  • Dergi Adı: FIBERS AND POLYMERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2236-2246
  • Anahtar Kelimeler: Osteomyelitis, Vancomycin, Poly(epsilon-caprolactone), Wet spinning, Fiber, IN-VITRO, BIODEGRADABLE POLY(EPSILON-CAPROLACTONE), DRUG-DELIVERY, RELEASE, MICROPARTICLES, FORMULATION, INFECTION
  • Hacettepe Üniversitesi Adresli: Evet

Özet

In this study, it was aimed to develop drug eluting biodegradable and biocompatible bone supportive fibers and films, which can locally be applicable on the infected bone defect area for osteomyelitis treatment. For this purpose, vancomycin (Vm) loaded gelatin (G) microspheres were prepared and dispersed into poly(epsilon-caprolactone) (PCL) solution and then the suspension was processed as films and fibers by solvent casting and wet spinning techniques, respectively. The mean particle size distribution and morphology of Vm loaded G microspheres were characterized by laser diffraction method and scanning electron microscopy, respectively. In vitro Vm release profiles and release kinetics from microsphere, fiber and film formulations were investigated. In vitro biodegradation properties of fiber and film formulations were examined in both hydrolytic and enzymatic media during 25 days period. The cytotoxicity of Vm eluting films and fibers were tested on L929 cells by MTT assay. Presence of PCL in film and fiber formulations retarded the release of Vm from microspheres and provided long term sustained release. Vm eluting films and fibers exhibited strong antibacterial properties against Staphylococcus aureus and Staphylococcus epidermidis.