Purification of immunoglobulin G from human plasma by metal-chelate affinity chromatography


OZKARA S., Yavuz H. , DENIZLI A.

JOURNAL OF APPLIED POLYMER SCIENCE, cilt.89, ss.1567-1572, 2003 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 89 Konu: 6
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1002/app.12312
  • Dergi Adı: JOURNAL OF APPLIED POLYMER SCIENCE
  • Sayfa Sayıları: ss.1567-1572

Özet

A new group of specific-affinity beads with metal chelates as ligands and synthetic polymer beads as support matrices was prepared and tested. This study focused on developing metal-chelated poly(hydroxyethyl methacrylate) (PHEMA) gel beads in a spherical form (100-150 mum in diameter) for the separation of immunoglobulin G (IgG) from human plasma. Crosslinked PHEMA gel beads were prepared by suspension polymerization. The metal-complexing ligand (L)-histidine was then immobilized by covalent binding onto these gel beads. Different transition-metal ions, including Zn(II), Ni(II), Co(II), and Cu(II), were chelated on these gel beads. An elemental analysis of immobilized L-histidine for nitrogen was estimated to be 401.9 mumol of (L)-histidine/g of PHEMA. The nonspecific IgG adsorption onto plain PHEMA gel beads was negligible (ca. 0.17 mg/g). Higher adsorption values (up to 3.5 mg/g) were obtained in which (L)-histidine-immobilized PHEMA gel beads were used from aqueous solutions. A remarkable increase in the IgG adsorption capacities were achieved from human plasma with L-histidine-immobilized gel beads (up to 44.8 mg/g). Further increases in the IgG adsorption capacities of the metal-chelated gel beads were observed when human plasma was used (up to 79.6 mg/g). The metal-chelate affinity gel beads allowed the one-step separation of IgG from human plasma. The recognition range of metal ions for surface histidines from human plasma followed this order: Cu(II) > Ni(II) > Zn(II) > Co(II). IgG molecules could repeatedly be adsorbed and desorbed with these metal-chelated PHEMA gel beads without a noticeable loss in their IgG adsorption capacity. (C) 2003 Wiley Periodicals, Inc.