Determination of thermodynamic and kinetic properties of biomolecules by mass spectrometry


Guelbakan B. , BARYLYUK K., ZENOBI R.

CURRENT OPINION IN BIOTECHNOLOGY, cilt.31, ss.65-72, 2015 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 31
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1016/j.copbio.2014.08.003
  • Dergi Adı: CURRENT OPINION IN BIOTECHNOLOGY
  • Sayfa Sayıları: ss.65-72

Özet

Over the past two decades, mass spectrometry (MS) has transformed the life sciences. The advances in understanding biomolecule structure and function by MS is progressing at an accelerated pace. MS has also largely been applied to study thermodynamic and kinetic structure of biomolecules. Herein, we highlight the recent discussions about native mass spectrometry and studies about determining stable gas phase structures, hydrogen/deuterium exchange studies about reaction kinetics and determination of binding constants of biomolecules with their ligands.