Determination of thermodynamic and kinetic properties of biomolecules by mass spectrometry

Guelbakan B., BARYLYUK K., ZENOBI R.

CURRENT OPINION IN BIOTECHNOLOGY, cilt.31, ss.65-72, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 31
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1016/j.copbio.2014.08.003
  • Dergi Adı: CURRENT OPINION IN BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.65-72
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Over the past two decades, mass spectrometry (MS) has transformed the life sciences. The advances in understanding biomolecule structure and function by MS is progressing at an accelerated pace. MS has also largely been applied to study thermodynamic and kinetic structure of biomolecules. Herein, we highlight the recent discussions about native mass spectrometry and studies about determining stable gas phase structures, hydrogen/deuterium exchange studies about reaction kinetics and determination of binding constants of biomolecules with their ligands.