Research Information System
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, vol.270, 2025 (SCI-Expanded, Scopus)
Diabetic ulcers are a serious complication of diabetes, often leading to reduced quality of life and, in many cases, limb amputation. These ulcers are characterized by impaired wound healing, primarily due to hyperglycemiainduced inflammation that disrupts normal cellular function. Given the limited effectiveness of conventional therapies, there is a critical need for alternative treatment strategies. This study investigated the therapeutic potential of photobiomodulation (PBM) using polychromatic light in the near-infrared range (600-1200 nm) on human cell types associated with diabetic wound healing, thereby extending previous research predominantly centered on monochromatic light sources. In vitro experiments were conducted using dermal fibroblasts, keratinocytes, and endothelial cells cultured under high-glucose conditions (25-150 mM) to simulate the diabetic environment. A glucose concentration of 150 mM was identified as optimal for modeling hyperglycemia, and PBM treatment was applied accordingly. The protocol consisted of irradiation at an intensity of 2.4 J/cm2 per minute, delivered from a distance of 20 cm for 3 min on alternate days over a 7-day culture period. Under these hyperglycemic conditions, PBM significantly enhanced the proliferation and migration of dermal fibroblasts and keratinocytes, restoring these functions to levels comparable to normoglycemic controls. Furthermore, PBM increased fibronectin and collagen synthesis in both control and hyperglycemic groups. In endothelial cells, PBM improved tube formation, which was otherwise impaired under elevated glucose levels. Overall, these results indicate that PBM using a polychromatic light source may serve as a promising adjunctive therapy for enhancing wound healing in diabetic ulcers.