Anchorage-dependent and suspended baby-hamster kidney cells on three-dimensional non-woven polyester fabric discs: Comparison of growth characteristics
Biotechnology and Applied Biochemistry, vol.30, no.1, pp.65-71, 1999 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 30 Issue: 1
- Publication Date: 1999
- Journal Name: Biotechnology and Applied Biochemistry
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.65-71
- Hacettepe University Affiliated: Yes
Abstract
Two types of baby-hamster kidney (BHK) cell line, anchorage-dependent BHK-An32 and suspended BHK-An48 cells, were cultivated on non-woven polyester fabrics (NWPF) that were prepared from non-woven polyethylene teraphthalate sheets with different void fractions in three-dimensional configuration. The attachment and growth characteristics of both cell lines were investigated in stationary and submerged cultures, containing Dulbecco's modified Eagle's medium supplemented with fetal-calf serum. All cultures displayed rapid attachment (< 120 min) of inoculated cells to the discs. The growth of BHK cells on NWPF discs in stationary cultures was similar to that observed in tissue-culture dishes; however, the denser discs showed better results in terms of final cell density, especially in the case of BHK-An48 cells. Spinner-flask cultures indicated that higher cell yields were obtained than in stationary conditions. Microscopic observation showed that anchorage-dependent BHK-An32 cells spread through the interfibre spaces of NPWF discs to form three-dimensional structures whereas BHK-An48 cells proliferated on fibres.