Metal-chelated magnetic nanoparticles for protein C purification


ÇİMEN D. , BERELİ N. , DENİZLİ A.

SEPARATION SCIENCE AND TECHNOLOGY, vol.55, no.13, pp.2259-2268, 2020 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 55 Issue: 13
  • Publication Date: 2020
  • Doi Number: 10.1080/01496395.2019.1618327
  • Title of Journal : SEPARATION SCIENCE AND TECHNOLOGY
  • Page Numbers: pp.2259-2268

Abstract

In the study, magnetic poly(hydroxyethylmethacrylate-N-methacryloyl-(L)-histidine) (mPHEMAH) nanoparticles were prepared by miniemulsion polymerisation using N-methacryloyl-(L)-histidinemethylester as basic monomer. The specific surface area of the magnetic nanoparticles was found to be 1050 m(2)/g. Maximum Protein C adsorption capacites of the Ni(II), Cu(II) and Zn(II) ions chelated magnetic poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine) nanoparticles were calculated as 589.22, 436.33 and 388.15 mg/g nanoparticles, respectively. This work was focused on the purity of the Protein C by sodium dodecyl sulphate polyacrylamide gel electrophoresis yielding a satisfactory result of 92% purity. The reusabilitys of the Ni(II)-mPHEMAH, Cu(II)-mPHEMAH and Zn(II)-mPHEMAH nanoparticles were approximately 93.5%, 94% and 95% respectively.