Metal-chelated magnetic nanoparticles for protein C purification

ÇİMEN D., BERELİ N., DENİZLİ A.

SEPARATION SCIENCE AND TECHNOLOGY, vol.55, no.13, pp.2259-2268, 2020 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 55 Issue: 13
  • Publication Date: 2020
  • Doi Number: 10.1080/01496395.2019.1618327
  • Journal Name: SEPARATION SCIENCE AND TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Agricultural & Environmental Science Database, Analytical Abstracts, Applied Science & Technology Source, Biotechnology Research Abstracts, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Pollution Abstracts, DIALNET, Civil Engineering Abstracts
  • Page Numbers: pp.2259-2268
  • Hacettepe University Affiliated: Yes

Abstract

In the study, magnetic poly(hydroxyethylmethacrylate-N-methacryloyl-(L)-histidine) (mPHEMAH) nanoparticles were prepared by miniemulsion polymerisation using N-methacryloyl-(L)-histidinemethylester as basic monomer. The specific surface area of the magnetic nanoparticles was found to be 1050 m(2)/g. Maximum Protein C adsorption capacites of the Ni(II), Cu(II) and Zn(II) ions chelated magnetic poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine) nanoparticles were calculated as 589.22, 436.33 and 388.15 mg/g nanoparticles, respectively. This work was focused on the purity of the Protein C by sodium dodecyl sulphate polyacrylamide gel electrophoresis yielding a satisfactory result of 92% purity. The reusabilitys of the Ni(II)-mPHEMAH, Cu(II)-mPHEMAH and Zn(II)-mPHEMAH nanoparticles were approximately 93.5%, 94% and 95% respectively.