Research Information System
IEEE Sensors Journal, vol.23, no.11, pp.11566-11573, 2023 (SCI-Expanded)
In this study, Concanavalin A imprinted surface plasmon resonance (SPR) biosensor was prepared for the determination of Concanavalin A. Concanavalin A imprinted SPR biosensors were characterized by atomic force microscopy, ellipsometer and contact angle measurements. The kinetic studies were performed with pH scanning. For this purpose, Concanavalin A solutions were prepared with different pH buffers and were used for detection of Concanavalin A. The kinetic analysis of SPR biosensor was with Concanavalin A solutions ranging from 0.01 to 50 ng/mL. The limit of detection and limit of quantification was calculated 0.0011 ng/mL and 0.0038 ng/mL, respectively. Selectivity studies were conducted using SPR biosensors that were either Concanavalin A imprinted or non-imprinted, as well as competing molecules such as bovine serum albumin and myoglobin, which have a similar molecular structure. In order to determine Concanavalin A in clinical studies, kinetic analyzes were performed with concanavalin a solutions prepared in artificial plasma and jack bean solutions. The recovery of Concanavalin A was calculated using the obtained sensorgram data. Four consecutive analyses were performed on the same day to test the concanavalin A molecule’s reusability. In order to test the reusability of the biosensor at various points in time, kinetic analyses were also performed out at the first, second, fourth, and sixth months.