Quantitative Photoelectrochemical Detection of Biotin Conjugated CdSe/ZnS Quantum Dots on the Avidin Immobilized ITO Electrodes

Bas D., BOYACI İ. H.

ELECTROANALYSIS, vol.21, no.16, pp.1829-1834, 2009 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 16
  • Publication Date: 2009
  • Doi Number: 10.1002/elan.200904619
  • Journal Name: ELECTROANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1829-1834
  • Hacettepe University Affiliated: Yes

Abstract

Photocurrent generation from CdSe/ZnS (core/shell) quantum dots (QDs) in a photoelectrochemical cell was proposed to perform a bioaffinity biosensor in this study. The photocurrent of QDs is reversible and methylene blue as an electron transfer mediator causes a four-fold increase in the photocurrent. We further present quantitative photoelectrochemical detection of biotin conjugated QDs on the avidin immobilized ITO electrodes. A linear calibration graph was obtained in the range of 4 and 18 nM of biotin conjugated QDs with a coefficient of determination of 0.997. Results imply that QDs can be successfully used as photoelectroactive labels for the photoelectrochemical biosensor systems.