Osteogenic activities of MC3T3-E1 cells on heparin-immobilized poly(caprolactone) membranes
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, vol.26, no.3, pp.257-269, 2011 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 26 Issue: 3
- Publication Date: 2011
- Doi Number: 10.1177/0883911511406329
- Journal Name: JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.257-269
- Hacettepe University Affiliated: Yes
Abstract
The aim of this study was to investigate the effects of heparin on the activity of osteoblast-like cells seeded on poly(caprolactone) (PCL) membranes. The membranes were prepared by solvent-casting technique in similar to 150 mu m thickness. Then they were treated with 1,6-hexanediamine solution and functionalized with covalently bound heparin. The morphology, proliferation, and differentiation of MC3T3-E1 preosteoblasts on these membranes were investigated in vitro. The heparin functionalized PCL membranes, compared to non-functionalized membranes, significantly stimulated osteoblast proliferation. The Scanning electron microscope images confirmed the stimulative effect of covalently bound heparin on the osteoblast-like cell proliferation. The alkaline phosphatase and osteocalcin levels for cells proliferated on heparin containing PCL membranes were higher than that of nonfunctionalized membranes.