PHEMA based composite cryogels with loaded hydrophobic beads for lysozyme purification

TÜRKMEN D., DENİZLİ A.

COLLOIDS AND SURFACES B-BIOINTERFACES, cilt.123, ss.859-865, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 123
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.colsurfb.2014.10.034
  • Dergi Adı: COLLOIDS AND SURFACES B-BIOINTERFACES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.859-865
  • Anahtar Kelimeler: Cryogels, Particle loading, Affinity chromatography, Protein purification, Lysozyme purification, POLY(HYDROXYETHYL METHACRYLATE) CRYOGEL, AFFINITY-CHROMATOGRAPHY, HUMAN SERUM, SUPERMACROPOROUS CRYOGELS, IMMUNOGLOBULIN PRODUCTION, PROTEIN CHROMATOGRAPHY, ALBUMIN DEPLETION, CONCANAVALIN-A, ADSORPTION, NANOPARTICLES
  • Hacettepe Üniversitesi Adresli: Evet

Özet

The purpose of this study is to synthesize megaporous cryogel loaded with hydrophobic affinity beads which can be utilized for the purification of lysozyme from chicken egg-white. N-methacryloyl-(L)-tryptophan methylester (MATrp) was used as the hydrophobic ligand. In the first step, poly(glycidyl methacrylate-N-methacryloyl-(L)-tryptophan methyl-ester) [PGMATrp] beads (2.2 mu m in diameter) were produced by dispersion polymerization. In the second step, the poly(2-hydroxyethyl methacrylate) (PHEMA),cryogel loaded with PGMATrp beads [PHEMA/PGMATrp composite cryogel] was polymerized initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) at -12 degrees C. Lysozyme adsorption capacity of the PHEMA/PGMATrp composite cryogel (332.7 mg/g polymer) was improved significantly due to the loading of PGMATrp beads into the cryogel structure. The hydrophobic MATrp comonomer played a vital role in this binding mechanism. The PHEMA/PGMATrp composite cryogel could be used many times without decreasing the lysozyme adsorption amount significantly. The main advantage of the PHEMA/PGMATrp composite cryogel is the high adsorption capacity. (C) 2014 Elsevier B.V. All rights reserved.