Determination of poly-beta-hydroxybutyrate (PHB) production by some Bacillus spp.

Yilmaz M., SORAN H., BEYATLI Y.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, vol.21, no.4, pp.565-566, 2005 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 4
  • Publication Date: 2005
  • Doi Number: 10.1007/s11274-004-3274-1
  • Journal Name: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.565-566
  • Keywords: Bacillus, bioplastic, isolation, PHB, soil, POLYHYDROXYALKANOATES, BACTERIA
  • Hacettepe University Affiliated: Yes

Abstract

In this study, 29 strains of the genus Bacillus were isolated from different soil samples which were taken from grasslands of Ankara, Turkey and were identified as B. brevis, B. sphaericus, B. cereus, B. megaterium, B. circulans, B. subtilis, B. licheniformis and B. coagulans. Two strains, B. sphaericus ATCC 14577 and B. subtilis ATCC 6633 were also included in this study. Poly-beta-hydroxybutyrate (PHB) production by these strains was determined by the spectrophotometric method, and it was found that PHB production ranged from 1.06-41.67% (w/v) depending on the dry cell weight. The highest PHB production and productivity percentage was found in B. brevis M6 (41.67% w/v).