Media optimization for xerotolerant yeasts in a model system


Senses S., Ozbas Z.

ARCHIV FUR LEBENSMITTELHYGIENE, vol.55, no.1, pp.19-22, 2004 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 55 Issue: 1
  • Publication Date: 2004
  • Journal Name: ARCHIV FUR LEBENSMITTELHYGIENE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.19-22
  • Hacettepe University Affiliated: No

Abstract

A study was undertaken to evaluate the recovery performances of Malt Extract Agar (MEA) (reference medium) and five selective media for three xerotolerant yeasts. These media are Malt extract Yeast extract 50 % Fructose Glucose (MY 50FG) agar, Malt extract Yeast extract 40 % Sucrose (MY 40S) agar, Malt extract Yeast extract Glucose 0.8 % Peptone (MYG 0.8 %P) agar, Malt extract Yeast extract Glucose 0.1 % Peptone (MYG 0.1 %P) agar, Potato Dextrose Agar 50 % Sucrose Glucose (PDA 50SG). Zygosaccharomyces rouxii 0487, Zygosaccharomyces bailii 0488, Saccharomyces cerevisiae 2359 were used as test strains. Water activity (a(w)) values of the media were found to be ranging between 0.89 and 0.99. While MYG 0.8 % P medium was determined to give the highest recovery for the Z. rouxii strain, MY 40S medium was found superior to the other media for the Z. bailii strain. On the other hand, for the S. cerevisiae strain no colony formation was observed on MY 50FG medium and the highest recovery was observed on MEA, which was also used as reference medium. As the results were statistically evaluated, the difference between MEA and MY 40S was found as not significant (p > 0.05). Also, the difference between MYG 0.1 % P and MYG 0.8%P was determined to be not significant at p less than or equal to 0.05 significance level. According to the subjective evaluations concerning size, colour and appearance of colonies, ease of counting and length of incubation period permitting visible colony growth, MY 40S medium was found superior to the other four media.