Research Information System
10th European Meeting on Viral Zoonoses, Lisbon, Portugal, 23 - 26 September 2023, pp.79, (Summary Text)
In recent years, there has been an increasing trend towards utilizing next generation sequencing (NGS) techniques to obtain viral sequences. Understanding the viral diversity of vectors such as ticks, by using NGS is one of the common approaches. Due to the potential of ticks to transmit both known and unknown viruses to human or animals, understanding the viral diversity in these animals is crucial. Thus, here we examined the RNA virome of two tick species collected from cattle animals from Eastern Trace in Turkey and providing preliminary results. The virus families obtained from virome analysis revealed that there is family-level clustering into three groups. Group (I) comprises as the families that have emerging viruses or important for one-health perspective. While Phenuiviridae for Phlebovirus as the second most prevalent (corresponds to the highest number of pools had this virus in biggest proportion of total reads) virus in tick pools and Nairoviridae for Crimean-Congo Hemorrhagic Fever virus as Europe lineage I for emerging viruses, Flaviviridae for Pestivirus that is known to be pathogenic for cattle animals as an important virus for veterinary medicine. Group (II) consists of families that are derived from environmentally such as from plants, fungi, or other parasites that ticks carry, and these families are: Botourmiaviridae, Mitoviridae, Narnaviridae, Partitiviridae, Picornaviridae, Tombusviridae, Totiviridae, Virgaviridae. Group (III) has families that known to be insect pathogens, and as expectedly, Mivirus from Chuviridae was the most prevalent. This group also had other families such as Hypoviridae and Rhabdoviridae. Our findings also contributed novelsequences for unclassified viruses in Bunyavirales such as Bole-tick virus-like or Liman-tick virus-like sequences. Furthermore, we obtained complete M- and L- segment sequences for a potential novel virus that may belong to the unclassified Bunyavirales.