Affinity based and molecularly imprinted cryogels: Applications in biomacromolecule purification


Andac M., Galaev I. Y., DENİZLİ A.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, cilt.1021, ss.69-80, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1021
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.jchromb.2015.09.034
  • Dergi Adı: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.69-80
  • Anahtar Kelimeler: Affinity purification, Molecularly imprinted cryogels, Biomacromolecules, METHACRYLATE) BASED CRYOGEL, CIBACRON BLUE F3GA, COMPOSITE CRYOGEL, MONOLITHIC CRYOGELS, IMMUNOGLOBULIN-G, PLASMID DNA, SUPERMACROPOROUS CRYOGELS, ALBUMIN DEPLETION, CONCANAVALIN-A, ADSORPTION PERFORMANCE
  • Hacettepe Üniversitesi Adresli: Evet

Özet

The publications in macro-molecularly imprinted polymers have increased drastically in recent years with the development of water-based polymer systems. The macroporous structure of cryogels has allowed the use of these materials within different applications, particularly in affinity purification and molecular imprinting based methods. Due to their high selectivity, specificity, efficient mass transfer and good reproducibility, molecularly imprinted cryogels (MICs) have become attractive for researchers in the separation and purification of proteins. In this review, the recent developments in affinity based cryogels and molecularly imprinted cryogels in protein purification are reviewed comprehensively. (C) 2015 Elsevier B.V. All rights reserved.