Structural characterization of a variety of spider silks from Turkey using different biophysical techniques

DE S., Haman Bayari S., TÜRKEŞ T., MERGEN Y. O., Çelik Ö., Bütün V., ...Daha Fazla

SPECTROSCOPY-BIOMEDICAL APPLICATIONS, cilt.25, ss.155-167, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 25
  • Basım Tarihi: 2011
  • Doi Numarası: 10.3233/spe-2011-0495
  • Dergi Adı: SPECTROSCOPY-BIOMEDICAL APPLICATIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.155-167
  • Anahtar Kelimeler: Spider silk, XRD, FTIR, scattering methods, SEM, mechanical testing, SECONDARY STRUCTURE ESTIMATION, CLAVIPES DRAGLINE SILK, X-RAY-SCATTERING, STATE C-13 NMR, VIBRATIONAL ANALYSIS, SPECTROSCOPY, PROTEINS, ANGLE, POLYPEPTIDES, CONFORMATION
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Various experimental methods (XRD, FTIR, SAXS, DLS and SEM) were used to investigate structures of the silk samples weaved by spiders in Turkey. Silk samples were collected from the natural habitats belonging to the spider species (i.e., Near Salt Lake/Sereflikochisar,. Ivriz Dam/Eregli-Konya, Karatas Lake/Burdur and several locations in Black Sea Region of Turkey). Among all collected species, Araneidae (Araneus angulatus, Argiope bruennichi, Argiope lobata, Larinoides cornutus), Eresidae (Eresus cinnaberinus), Agelenidae (Agelena labyrinthica), Oecobidae (Uroctea durandi), Miturgidae (Cheiracanthium erraticum), Salticidae (Heliophanus flavipes, Philaeus chrysops), Theridiidae (Steatoda paykulliana) and Gnaphosidae were found significant amongst other spider species. Structural differences and similarities of these species were reported. Diameter range of draglines, inter crystallite distance of nano-size structures, mean size range of nano-structure aggregations, crystallite size range, information about protein content were obtained. The mechanical properties of some silk fibers were also investigated.