Platelet-rich fibrin (PRF) is an endogenous coagulation system product used successfully. Ankaferd Blood Stopper (ABS) is a substance obtained from various plants and used as a hemostatic agent. The aim of this study is to show the effects of ABS on the PRF membrane, the durability and resorption time of which are being discussed. For this purpose, blood samples were taken from 10 volunteers and 20 PRF membranes obtainded. 10 PRF membranes were kept in ABS for 10 minutes and dissolved in trypsin (PRF+ABS group). The other 10 PRF membranes was dissolved in trypsin without any treatment (PRF group) and biodegradation was achieved. Percentage values of the remaining material were obtained at 24-hour intervals. The remaining mass percentage values of the PRF group at all time intervals were found to be statistically significantly higher than the values of the PRF + ABS group (p <0.05). In addition, the remaining mass percentage values of both groups were shown to decrease statistically significantly in each 24-hour time interval (p <0.05). In addition, the remaining mass percentage values of both groups were found to be statistically significantly reduced in each 24-hour time interval (p <0.05). These results show that the ABS loaded PRF membrane was faster in the trypsin and the material obtained with ABS is more weak. But; it brings to mind the idea that the PRF membrane can be a good carrier for ABS. It is thought that it can accelerate tissue healing by rapidly introducing PRF metabolites and ABS metabolites that have been shown to be beneficial for tissue healing.

Keywords: ankaferd blood stopper, biodegradation, platelet-rich fibrin, trypsin.