Detection of irradiated wheat using the electron paramagnetic resonance spin probe technique

Dadayli D., Sunnetcioglu M., Koksel H., Celik S.

CEREAL CHEMISTRY, vol.74, no.4, pp.375-378, 1997 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 74 Issue: 4
  • Publication Date: 1997
  • Doi Number: 10.1094/cchem.1997.74.4.375
  • Journal Name: CEREAL CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.375-378
  • Hacettepe University Affiliated: Yes

Abstract

In this study, the electron paramagnetic resonance (EPR) spin probe technique is applied as a detection method in the differentiation of irradiated and unirradiated wheat seeds. Two wheat cultivars, Kunduru and Bezostaya, were used. Aqueous solutions of 4-hydroxy-TEMPO (TANOL) spin probe prepared with a line-broadening agent, potassium ferricyanide, were used in all experiments. The EPR spectra of the samplea were recorded against time. A decrease in the signal intensity and a change in the shape of the intensity-time curve (rehydration curve) were observed, depending on the applied level of irradiation. The ratio of the lipid and aqueous regions at the high field (m(I) = -1) line changes, depending on the dose of irradiation.