Zr-89-labelled Obinutuzumab: a potential immuno-PET radiopharmaceutical

Sarcan E. T., Paisey S., Ruthardt M., ÖZER A. Y., Marshall C., Hartman N.

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, cilt.331, ss.5507-5516, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 331
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s10967-022-08614-5
  • Dergi Adı: JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Analytical Abstracts, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, INSPEC, Metadex, Pollution Abstracts, Public Affairs Index, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.5507-5516
  • Anahtar Kelimeler: Zirconium-89, Tociluzumab, Obinutuzumab, Burkitt's Lymphoma, CD20, NHL, MONOCLONAL-ANTIBODIES, BIFUNCTIONAL CHELATE, EMISSION TOMOGRAPHY, ZR-89, ZIRCONIUM-89, FRAGMENTS, CD20, CANCER
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Obinutuzumab (Obi) is a clinically approved a next-generation monoclonal antibody (mAb), showing specific binding to the CD20 B-cell surface markers which is a key target for B-cell malignancies. In this study, Zr-89-labelling Obi was optimised and the binding of the labelled agent was validated in an in-vitro model. Obi was radiolabelled with Zr-89 with several different ratios to optimise the (mAb: DFO-Bz-NCS: Zr-89) ratio with a small amount of it. The reason to employ the antibody as low quantity as possible in radiolabelling studies was the very high cost of antibodies. The radiolabelling studies were followed by in-vitro studies that would demonstrate the affinity of the formulations to the cells of CD20(+). Proving the specificity of Zr-89-Obi may be beneficial in diagnosing and monitoring CD20(+) tumours. Obi's long half-life and high sensitivity to CD20 are important in this area.